Pipetting correctly.

I am going to put my ego to one side, and just be honest myself.. I can not pipette at all! yeah sure I have used a micropipette before in laboratory settings but all this time I was pipetting incorrectly and no one told me...(until my training officer told me to review my pipetting skills)..

Anyway I found 2 useful YouTube videos:
https://www.youtube.com/watch?v=uEy_NGDfo_8&t=9s
https://www.youtube.com/watch?v=05oolv_y4Pk 

The videos shows the common "Don't" and "Do's" when pipetting. Visually watching someone pipette, I feel has helped me better my pipetting skills in a way.

Things I did not know before: 
When dispensing liquid into a well, the tip needs to be at an angle (10-45°).


Plunger needs to be carefully moved back to its resting position (and not too fast!).

Pipette tip should not be immersed too far into the liquid (immersion to 2-3mm depth is suitable).

If there is remaining liquid in the pipette tip, depress the plunger to the second stop position.




Comments

Post a Comment

Popular posts from this blog

Passed IBMS Verification :)

 I can finally proudly say that I have passed my IBMS verification.. yikes what a journey it has been... all my hard work has really paid off! from writing my evidences to training in the lab - I can confidently say that I will not do my specialist training in immunology.. however it has been an experience to say the least. If anyone needs advice or help with evidences/portfolio please do not hesitate to ask :) OR if you want to chat...
Read more

Barriers To CPD By (Tahmina Hussain) - Workshop

Image
  I have tried to attend The Biomedical Scientist Live virtual event.. although I was not able to attend the event as I am not a IBMS member.... instead, I watched the recording of the event (free of charge!) on IBMS Support Hub. In the session, examples of CPD were discussed throughout the workshop and reflective writing was also talked about in terms of how it can be challenging to get in the habits of writing reflectively as a Scientist.  It was also mentioned that the IBMS CPD scheme is not used by all members... I never even knew that such scheme existed..if I am being honest!  I should start using the CPD scheme!
Read more

ANCA - Associated Vasculitis Case Study

Patient Story: 70 years old male Upon seeing the GP (On  01.05.20 ), the patient presented with persistent cough,  rash, and shortness of breath, and feeling unwell. Previous medical history: asthma, nose bleeds, recurrent, nasal congestion, fatigue and rash since October 2019.  Patient was referred to a respiratory consultant (as an outpatient) on the  14.05.20 .  The patient was admitted to K hospital, with collapsed lung. Then transferred to another hospital as an inpatient on  21.05.20  with rapidly progressive pulmonary renal syndrome. On the  23.05.20  the patient began haemodialysis, given 3 pulses of IV methylprednisolone soon after and started on cyclophosphamide on  27.05.20 . GP Results:  Urea (2.5-7.0 mmol/L)   Creatinine (60-106 umol/L)   EGFR (>60 mL/min/1.73m2)   CRP (0-10 mg/L)  10.12.18: 5.4  67  >60   01.05.20 7.1 111   58 ...
Read more