Pipetting correctly.
I am going to put my ego to one side, and just be honest myself.. I can not pipette at all! yeah sure I have used a micropipette before in laboratory settings but all this time I was pipetting incorrectly and no one told me...(until my training officer told me to review my pipetting skills)..
Anyway I found 2 useful YouTube videos:
https://www.youtube.com/watch?v=uEy_NGDfo_8&t=9s
https://www.youtube.com/watch?v=05oolv_y4Pk
The videos shows the common "Don't" and "Do's" when pipetting. Visually watching someone pipette, I feel has helped me better my pipetting skills in a way.
Things I did not know before:
When dispensing liquid into a well, the tip needs to be at an angle (10-45°).
Anyway I found 2 useful YouTube videos:
https://www.youtube.com/watch?v=uEy_NGDfo_8&t=9s
https://www.youtube.com/watch?v=05oolv_y4Pk
The videos shows the common "Don't" and "Do's" when pipetting. Visually watching someone pipette, I feel has helped me better my pipetting skills in a way.
Things I did not know before:
When dispensing liquid into a well, the tip needs to be at an angle (10-45°).
Plunger needs to be carefully moved back to its resting position (and not too fast!).
Pipette tip should not be immersed too far into the liquid (immersion to 2-3mm depth is suitable).
If there is remaining liquid in the pipette tip, depress the plunger to the second stop position.
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